AJCL3022 - CME/CMLE - Utilization of a Vanadate Oxidation Conjugated Bilirubin Assay to Reduce Test Cancellations due to Hemolyzed Specimens in Pediatric Patients-Utilization of a Vanadate Oxidation Conjugated Bilirubin Assay to Reduce Test Cancellations due to Hemolyzed Specimens in Pediatric Patients

Utilization of a Vanadate Oxidation Conjugated Bilirubin Assay to Reduce Test Cancellations due to Hemolyzed Specimens in Pediatric Patients

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The study investigated the use of a vanadate oxidation method for measuring conjugated bilirubin in pediatric patients to reduce test cancellations due to hemolyzed specimens. The vanadate method showed robust performance against hemolysis, with no interference observed up to an H-index of 1,300. Implementation of the vanadate assay significantly decreased the proportion of pediatric tests cancelled due to hemolysis compared to the diazo method. The study found that using the vanadate method resulted in a notable decrease in test cancellations, particularly in neonatal and infant populations. The analytical validation studies of the vanadate assay demonstrated good performance, with a positive bias of 1.27 compared to the diazo method. Adjustments were made to ensure the interchangeability of methods in the laboratory. The adoption of the vanadate method proved to be effective in reducing hemolysis-related test cancellations in pediatric patients, allowing for better patient care. The study highlighted the importance of accurate bilirubin measurements in pediatric patients and the impact of method selection on test cancellations. Further research and in-house correlation studies are recommended to ensure the accuracy and reliability of new testing methods.

Keywords

vanadate oxidation method

conjugated bilirubin

pediatric patients

test cancellations

hemolyzed specimens

H-index

analytical validation studies

neonatal and infant populations

method interchangeability

hemolysis-related test cancellations