AJHE2109 - CME/CMLE - A Comparative Evaluation of Capillary Electrophoresis, Cation-Exchange High-Performance Liquid Chromatography, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Screening of Hemoglobin Variants-A Comparative Evaluation of Capillary Electrophoresis, Cation-Exchange High-Performance Liquid Chromatography, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Screening of Hemoglobin Variants

A Comparative Evaluation of Capillary Electrophoresis, Cation-Exchange High-Performance Liquid Chromatography, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Screening of Hemoglobin Variants

Back to course

Pdf Summary

The study evaluated three methods, capillary electrophoresis (CE), cation-exchange high-performance liquid chromatography (HPLC), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), for screening hemoglobin (Hb) variants in southern China. CE method was found to detect the most Hb variants, including the top 10 prevalent in the region, compared to the other methods. Variant II Turbo 2.0 and QuanTOF had varying detection rates, with QuanTOF having a mass difference detection limit of approximately 11 to 20 Da. While the CE method was effective in detecting most variants, MALDI-TOF MS was deemed suitable as an auxiliary method due to its limitations in detecting all variants accurately. The study highlighted the importance of using a combination of methods for comprehensive Hb variant screening. The ten most common Hb variants in southern China were identified, with Hb E being the most prevalent. The study emphasized the need for accurate detection and recognition of Hb variants due to their clinical significance in inherited disorders. Ultimately, the study recommended CE as the preferred method for initial screening in clinical laboratories in southern China, with MALDI-TOF MS serving as a complimentary tool.

Keywords

capillary electrophoresis

cation-exchange high-performance liquid chromatography

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

hemoglobin variants

southern China

Variant II Turbo 2.0

QuanTOF

mass difference detection limit

Hb E

inherited disorders