AJHE2205 - CME/CMLE - The Effectiveness of DualStaining Immunohistochemistry in the Detection of Mantle Cell Lymphoma in the Bone Marrow-The Effectiveness of DualStaining Immunohistochemistry in the Detection of Mantle Cell Lymphoma in the Bone Marrow

The Effectiveness of DualStaining Immunohistochemistry in the Detection of Mantle Cell Lymphoma in the Bone Marrow

Pdf Summary

The study evaluates the effectiveness of dual-staining immunohistochemistry (IHC) in detecting mantle cell lymphoma (MCL) in bone marrow samples. Traditionally, detecting MCL with single stains can be challenging, especially in cases of low-level disease. The research utilized cyclin D1/CD79a and PAX5/CD5 dual stains and compared them with single stains and flow cytometry. The findings suggest that dual stains had a higher efficacy in detecting MCL cells compared to single stains, particularly in cases with minimal disease burden. The dual stains detected MCL cells in up to 95.6% of cases positive by flow cytometry. Furthermore, the study highlighted the significance of dual stains in cases where bone marrow aspirates are inadequate for flow cytometry analysis. The dual-staining IHC appeared comparable to flow cytometry and superior to single IHC staining in specific recognition of MCL cells. The study recommends incorporating dual staining methods alongside traditional tests for diagnosing MCL, especially in cases with minimal disease involvement or when adequate bone marrow aspirates are unavailable for other analyses. Overall, the research emphasizes the value of dual-staining IHC in detecting MCL, suggesting its utility in cases with low-level disease burden and underscoring its importance for accurate diagnosis and subsequent management of MCL.

Keywords

dual-staining immunohistochemistry

mantle cell lymphoma

bone marrow samples

cyclin D1/CD79a

PAX5/CD5

flow cytometry

minimal disease burden

diagnosis

management

MCL cells