LQCL2406 - CMLE - The Importance of Lymphocyte Gate Purity in T-, B-, and NK-Cell Immunophenotyping-The Importance of Lymphocyte Gate Purity in T-, B-, and NK-Cell Immunophenotyping

The Importance of Lymphocyte Gate Purity in T-, B-, and NK-Cell Immunophenotyping

Pdf Summary

The document discusses the importance of lymphocyte subset immunophenotyping in clinical monitoring, especially for patients with HIV, immunodeficiency, and post-transplant evaluation. It details the challenges faced in accurately quantifying T, B, and NK cells in peripheral blood due to overlap between lymphocytes and monocytes. Gating strategies using CD45 and side scatter are explored to improve identification of individual cell populations. The addition of a monocyte marker like CD14 is suggested to exclude monocytes and enhance the accuracy of lymphocyte subset enumeration. The history and evolution of flow cytometry in identifying leukocyte populations are highlighted, from early light-scattering techniques to the development of monoclonal antibodies targeting specific cell markers. Furthermore, the document emphasizes the need for implementing quality control parameters to ensure accurate lymphocyte subset analysis. It concludes by suggesting that continual refinement of assays, like adding a monocyte-specific marker, is essential for accurate reporting and future improvements in lymphocyte subset analysis.

Keywords

lymphocyte subset immunophenotyping

clinical monitoring

HIV

immunodeficiency

post-transplant evaluation

T cells

B cells

NK cells

flow cytometry

quality control parameters