LQHS2202 - CMLE - CELL BLOCK PREPARATION IN CYTOPATHOLOGY: ALTERNATIVES FOR THE MOLECULAR AGE-CELL BLOCK PREPARATION IN CYTOPATHOLOGY: ALTERNATIVES FOR THE MOLECULAR AGE

CELL BLOCK PREPARATION IN CYTOPATHOLOGY: ALTERNATIVES FOR THE MOLECULAR AGE

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The document discusses cell block preparations in histology and cytology, emphasizing the importance of molecular biomarker testing. A case study involving a patient with suspected metastatic lung carcinoma is presented, highlighting the use of various cell block preparation techniques and molecular testing to confirm the diagnosis. The potential sources of contamination in cell blocks, specifically from the use of plasma thrombin, are explored, leading to a switch to alternative methods like HistoGel and collodion bag. The significance of cell block preparation in diagnosing and treating non-small cell lung carcinomas, particularly for next-generation sequencing (NGS) testing, is emphasized. Recommendations for best practices in cell block preparation for molecular testing are provided, emphasizing the need for further evaluation and potential changes in laboratory protocols. The advantages and disadvantages of different cell block preparation methods are compared, with a focus on reducing the risk of DNA contamination. The importance of collaboration between cytotechnologists and cytopathologists in evaluating and implementing these changes is emphasized. The summary emphasizes the need for standardized guidelines in cell block preparation methods for molecular testing, with a focus on minimizing the risk of contamination and ensuring accurate diagnostic results.

Keywords

histology

cytology

cell block preparation

molecular biomarker testing

metastatic lung carcinoma

plasma thrombin contamination

HistoGel

collodion bag

non-small cell lung carcinomas

next-generation sequencing (NGS) testing