LQHS2303 - CMLE - SOLID TUMOR MOLECULAR TESTING: FROM HISTOLOGY TO MOLECULAR DIAGNOSTICS-SOLID TUMOR MOLECULAR TESTING: FROM HISTOLOGY TO MOLECULAR DIAGNOSTICS

SOLID TUMOR MOLECULAR TESTING: FROM HISTOLOGY TO MOLECULAR DIAGNOSTICS

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The document discusses the role of histotechnology in preparing solid tumor specimens for molecular testing, emphasizing the importance of proper handling for accurate results. It outlines the process from sample acquisition to nucleic acid extraction, quality testing, and storage of DNA/RNA for long-term preservation. The case study of an 82-year-old woman with metastatic melanoma highlights the use of molecular testing to guide treatment decisions. Techniques such as paraffin microdissection and paraffin core collection are explained in detail. The document also addresses the significance of RNA sequencing in identifying fusion genes in solid tumors. Lastly, the importance of quality testing before molecular analysis is stressed to ensure reliable results. Molecular pathology is highlighted as a key aspect in diagnosing and treating diseases like cancer, hereditary disorders, and infectious diseases. The document also includes CMLE questions to reinforce understanding of the material covered.

Keywords

histotechnology

solid tumor specimens

molecular testing

sample acquisition

nucleic acid extraction

DNA storage

RNA storage

metastatic melanoma

paraffin microdissection

RNA sequencing