LQHS2402 - CMLE - BONES AND BEYOND: A COMPREHENSIVE LOOK INTO DECALCIFICATION METHODS IN THE PATHOLOGY LABORATORY-BONES AND BEYOND: A COMPREHENSIVE LOOK INTO DECALCIFICATION METHODS IN THE PATHOLOGY LABORATORY

BONES AND BEYOND: A COMPREHENSIVE LOOK INTO DECALCIFICATION METHODS IN THE PATHOLOGY LABORATORY

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The document explores the topic of histology decalcification methods, particularly in bone samples. It covers the learning objectives related to understanding different decalcification techniques and their impact on patient care. A case study of a 6-year-old with a suspected bone lesion is detailed, emphasizing the importance of proper decalcification for diagnosis. Various decalcification agents like EDTA, HCl, and formic acid are compared for their advantages and disadvantages. The optimal choice depends on factors like tissue type and urgency of results. The text discusses the challenges and importance of balancing the need for rapid results with the accuracy of diagnostic testing, especially in cases requiring molecular analysis. Emerging technologies, such as microwave-assisted decalcification and laser-assisted microdissection, are highlighted for their potential to improve tissue preservation and analysis. The reconceptualization of decalcification methods aims to enhance diagnostic accuracy and personalized patient care. The major takeaway is the critical role of selecting the right decalcification method tailored to specific sample needs in improving diagnostic outcomes and patient management.

Keywords

histology

decalcification methods

bone samples

learning objectives

decalcification techniques

patient care

case study

decalcification agents

tissue preservation

emerging technologies