LQCL2614 - CMLE - DNA Quantitation and Quality Assessment: Fluorometric, Spectrophotometric, and Real-Time PCR-Based Methods in Pharmacogenetic Workflows

Course Description

LabQ 2026 Clinical Laboratory: Molecular Pathology

Faculty/Authors

Dean Ihemesie¹; Julianna Orlando²; Dan Wiener³;
Bronwyn Ramey, PhD, MB(ASCP), HCLD(ABB)⁴¹Incite Health
Doylestown, Pennsylvania
²Onetenacity
Windsor
Ontario, Canada
³Advanced Genetics Lab
Monroe, Connecticut
⁴Phoenix Laboratory Consulting
Louisville, Kentucky

CMLE Credit: 2.0

Estimated Completion Time: 2 hour

Format: Online Educational Activity and Post Exam

Instructions

To claim CMLE credit for the exercise, do the following:

Review the Technical Considerations.
Click Go to Course to view an overview of the modules in this course.
Click Access to begin the course.
Review the Educational Activity.
Complete and submit the Post Exam.
Submit the course Evaluation to register your credit.

Faculty Disclosures
The faculty have no relevant financial relationships with commercial interests to disclose.

Technical Considerations

Release Date: 3/31/2026
Review Date:
Expiration Date: 12/31/2028

Course Objectives

Following completion of this activity, you will be able to

differentiate the principles of fluorometric, spectrophotometric, and real-time polymerase chain reaction (PCR)-based DNA quantitation;

identify the strengths and weaknesses of fluorometric, spectrophotometric, and real-time PCR-based approaches for clinical laboratory workflows;

apply quality control (QC) cutoffs for purity, concentration, and amplifiability in DNA analysis;

interpret real-world discrepancies in DNA measurement across laboratories; and

select appropriate quantitation and QC methods for molecular testing.

Summary

Availability:

On-Demand

Credit Offered:

2 CMLE Credits